Phytochemical Studies and Evaluation of Antioxidant, Anticancer and Antimicrobial Properties of Conocarpus erectus L. Growing in Taif, Saudi Arabia

Abstract

The use of plants and their derived substances increases day by day for the discovery of new therapeutic agents owing to their versatile applications. Conocarpus erectus L. (family Combretaceae) is one of two species in the genus Conocarpus widely spread in Taif and other parts of the Kingdom of Saudi Arabia. It is used in some countries as folk remedy for anemia, catarrh, conjunctivitis, diabetes, diarrhea and fever. To our knowledge from literatures there is little work about phytochemical contents and biological activities of C. erectus which encouraged the authors to carry out this study. The four defatted methanol extracts of C. erectus different parts (leaves, stems, fruits and flowers) showed high free radical scavenging activity toward DPPH radical with SC 50 between 6.47-9.4 µg/ml. The nBuOH fractions obtained from successive fractionation of the four defatted methanol extracts had radical scavenging activity with SC 50 between 4.43-5.89 µg/ml higher than the EtOAc fractions (7.04-10.71 µg/ml). Due to the high antioxidant activity of the EtOAc and n-BuOH fractions, it was in vitro assayed toward two human cancer cell lines; HepG2 & MCF-7; using Sulphorhodamine-B assay method. The results showed that most fractions had cytotoxic activity with IC 50 < 20µg/ml which fall within the American Cancer Institute criteria. A preliminary antibacterial screening of the four defatted methanol extracts of the different parts toward five bacterial strains were done. Phytochemical investigation of the