Antibacterial activity of organic solvent fraction from Euphorbia supina

Abstract

In this study, the value of Euphorbia supinain antibiotics and disinfectants was investigated. E. supina was cultivated in Wonju, Gangwon-do, Republic of Korea, until August to November, 2013. After the harvest, E. supina was extracted with methanol. Extracts of MeOH were partitioned with different polaritic organic solvents (n-hexane, CHCl3,EtOAc, n-BUOH and distilled water fractions). The antibacterial activity of each fraction used disk diffusion. First, all fractions of E. supina extracts showed antibacterial activity against Gram positive bacteria. Especially, EtOAc fractions were 14±8 mm against Staphylococcus aureus and 10±0 mm against Bacillus subtilis. EtOAc fractions were partitioned into 6 sub-fractions by high performance liquid chromatography (HPLC). Each subfraction investigated the antibacterial activity by minimum inhibitory concentration (MIC). As the second result of subfraction, number 2 subfraction showed 2.5 mg/ml against S. aureus, 0.62 mg/ml against B. subtilis and number 5 subfraction showed 2.5 mg/ml against S. aureus, 0.62mg/ml against B. subtilis. Through this study, E. supina has utility as a natural pharmacological raw material for natural antibiotics and disinfectants. Key words: Euphorbia supina, antibacterial activity, antibiotics, disinfectant.