Phytochemical Screening, Total Phenolic, and Potential of Methanol Extract Musa balbisiana Colla as Antioxidant and Anti-Gout

Abstract

In this study, Musa balbisiana Colla seeds were extracted using Ultrasonic Assisted Extraction method. it was found that seeds extract of Musa balbisiana Colla contained alkaloid, phenolic, saponin, tannin and steroid glycoside compounds by phytochemical test. Antioxidant activity was determined by 1,1-diphenyl-2- picrylhydrazil and ferric reducing antioxidant power method while allopurinol was used to determine non uric acid capacity of Musa balbisiana Colla seeds extract. The total phenolic content of Musa balbisiana Colla seeds extract was 375mg/mg gallic acid. Antioxidant activity test was carried out using 1,1-diphenyl-2- picrylhydrazil method and ferric reducing antioxidant power method with the result in the form of Inhibitory Concentration 50. Inhibitory Concentration 50 value of Musa balbisiana Colla seeds extract was around (94.27±0.22) mg/L by 1,1-diphenyl-2- picrylhydrazil method which was classified as a very strong antioxidant activity. Inhibitory Concentration 50 value of Musa balbisiana Colla seeds extract using ferric reducing antioxidant power method was around (409.91±2.35)mg/L which was classified as very weak antioxidant activity. Anti-uric acid activity of Musa balbisiana Colla seeds was higher than the synthetic anti-uric acid drug allopurinol, which was around 38.27% and allopurinol was about 2.80%. These results illustrate that the seeds extract of Musa balbisiana Colla have the potential material as an ingredient in treating diseases caused by gout.