Abstract

Peronema canescens Jack, locally known as sungkai, is used as medicinal plant because it contains chemical compounds such as peronemins, catechol, quinic acid, isovanillic acid and guaiacol. The study was conducted to determine the chromatographic profile of the P. canescens leaves extract via liquid chromatography. It was found that the optimised mobile phase for thin layer chromatography (TLC) of P. canescens leaves was hexane: ethyl acetate: isopropanol (7:3:0.3). In addition, high performance liquid chromatography (HPLC) chromatograms (λ = 210 and 254 nm) of the methanol extract, run in 0.1% formic acid and acetonitrile, provided eight acceptable peaks.

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