Abstract
Background: Some Cola species are used in folklore medicine for the management of cancer related illnesses; thus, the objective of this work was to determine the phytochemical constituents, anti-proliferative and apoptotic activities of crude extract and fractions from the root bark of Cola rostrata on MCF-7 cells line. Methods: The root bark of Cola rostrate was collected, identified, cleaned, air dried, pulverized, extracted with methanol and fractionated into petroleum ether, chloroform, ethylacetate and butanol and these fractions were concentrated in vacuo. The MCF-7 cells lines were treated with 10 µg/ml of the crude extract and fractions of Cola rostrata. Results: The cells cycle progression showed that the crude extract had an apoptotic activity with 15.96 % of cells undergoing apoptosis as compared to 6.26 % of cells treated with DMSO, showing 9.70 % increase in population of apoptotic cells (P < 0.001). Also chloroform fraction treated MCF-7 cells gave 30.30 % proliferation which showed a significant decrease when it was compared with DMSO treated cells of 39.34 %, indicating 9.04 % reduction in proliferative cells (P < 0.01). Conclusion: These results provide evidence that Cola rostrata crude extract induces antiproliferative action, while the chloroform fraction induces apoptotic activity in MCF-7 cell line and could be used in the management of MCF-7 cell line ailments. Keywords: Cola rostrata, MCF-7, Flow cytometer, Apoptosis, Antiproliferation
Published Version
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