Phytochemical screening and the antioxidant, antibacterial and antifungal activities of aqueous extracts from the leaves of Salvia officinalis planted in Morocco

Abstract

The aqueous extract of the leaves of Salvia officinalis planted in Morocco is analyzed using different methods in order to study the different phytochemical components (phytochemical screening) and to test in parallel the antioxidant, antibacterial and antifungal activities. The main phytochemical components of salvia officinalis are: phenolics complex 13.87±1.96 mg E.AG/g, flavonoids 7.38±1.91mg E.Q/g and tannins 0.18±0.03 μg E.Cat/g, as well as different concentrations of saponins, alkaloids and mucilage. The antioxidant activity is achieved by four different methods: the DPPH test (IC-50 = 8.2 μg/ml higher than BHT (1.2 μg/ml)); the s-carotene decolorization test (the inhibition of oxidation of the aqueous extract (64.2%) was greater than the negative control (13. 87%)); the reducing power test (FRAP) (detection of a low reducing power of iron for the aqueous extract compared to quercetin) and the total antioxidant capacity (CAT), this test revealed an antioxidant capacity of 103.7±5.8 mg/g. The aqueous extract of salvia officinalis leaves showed antibacterial activity against four pathogenic bacteria: E. coli 57, K. pneumoniae, P. aeruginosa, S. aureus, noting MIC values of 0.25mg/ml; 0.125mg/ml; 0. The extract showed antifungal activity against two yeasts (S. sereveseae (0.25 mg/mlet C. albicans 0.25 mg/ml), using the agar diffusion method and the direct contact method.