Phytochemical screening and potential antioxidant of Amphora sp. in different extraction methods

Abstract

Character of Amphora sp., has a golden brown color with bioactives such as photosynthetic pigments which have important biological functions for health. This study aims to determine the method of Amphora sp. extraction which is more effective and efficient in producing qualitative and quantitative bioactive, as well as the best antioxidant activity. Amphora sp., extracted with absolute ethanol solvent using different extraction methods, namely sonication (SON), maceration (MAS), and sonication-maceration (SON-MAS). The sonication method showed more effective and efficient results with yield value. The results showed that the SON-MAS method produced the highest yield of 9.8% compared to MAS 8.71% and SON 8.47%. The highest chlorophyll content was seen in the MAS method, total chlorophyll 2.36 ± 0.07 mg/l. Phytochemical content of the SON-MAS and SON methods contained flavonoids, alkaloids, and triterpenoids, while the MAS method showed two compounds, namely alkaloids and triterpenoids. Quantitatively, the phenol and flavonoid content showed that the SON method had a higher value, namely phenol and flavonoids. The best DPPH free radical scavenging antioxidant activity was seen in the SON method with an IC50 value of 745.2 ppm. The extraction method by sonication is considered to have effectiveness and efficiency in producing bioactive Amphora sp.