Abstract
In the present study, antioxidant potential of the methanol and the ethyl acetate extracts of the seeds and pods of Calycotome villosa subsp. intermedia were evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay, reducing power and total antioxidant capacity. In DPPH scavenging assay, the IC50 value of the methanolic extract of seeds was found to be 0.20 mg/mL, the standard reference value for butylatedhydroxytoluene (BHT) is 0.19 mg/mL. Total antioxidant activity was also found to increase in a dose dependent manner. Moreover, Calycotome villosa extract showed strong reducing power. The total phenolic content in the extracts was determined using Folin-Ciocalteu reagent and their amounts ranged between 173.37 ± 0.02 to 332.23 ± 0.01 mg GA (Gallic Acid)/g of dry extract. The concentrations of flavonoids in the extracts varied from 5.02 ± 0.03 to 66.45 ± 0.01 Qu (Quercetin)/g of dry extract. Ethyl acetate extract of seeds of Calycotome villosa showed the highest phenolic, flavonoids concentration and the strongest antioxidant activity. The high contents of phenolic compounds indicated that these compounds contribute to the antioxidant activity. Furthermore, all extracts showed good antibacterial activity against Escherichia coli. Calycotome villosa subsp. intermedia can be regarded as promising candidate for natural plant sources of antioxidants with high value.
Highlights
Antioxidant research is an important topic in the medical field as well as in the food industry
Phytochemical screening The results of phytochemical screening of seeds and pods for the methanol and ethyl acetate extract of Calycotome villosa subsp. intermedia are given in the Table 1
The cyanidin test for flavonoids showed the presence of flavones in methanolic extract and the flavonols in ethyl acetate extract which is confirmed by a previous study, two flavonols no glycosylated were isolated from the seeds (El Antri et al, 2010)
Summary
Antioxidant research is an important topic in the medical field as well as in the food industry. Medicinal ones, have been extensively studied for their antioxidant activity in recent decades. Antioxidants from aromatic, spicy, medicinal, and other plants were studied to develop natural antioxidant formulations for food, cosmetic, and other applications (Miliauskas et al, 2004). Medicinal uses have been reported only for Calycotome villosa (Poiret) Link as antitumoral agent (Hartwell, 1982) and for the treatment of furuncle, cutaneous abscess and chilblain in the Sicilian folk medicine (Lentini et al, 1993). In previous phytochemical studies of Calycotome villosa subsp. The aims of this study were to screen for phytochemical constituents, determine the total phenolic and flavonoid contents, and evaluate in vitro antioxidant activity of seeds and pods extracts of Calycotome villosa subsp.
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