Phytochemical screening and antibacterial assay of the crude extract and fractions of Ferula oopoda

Shazia Iqbal

doi:10.19045/bspab.2019.80016

Abstract

Abstract The principal objective of the current study was to analyse phytochemical constituents and to determine the antimicrobial activity of the crude methanol extract and fractions of chloroform, ethyl acetate and hexane from the whole plant of Ferula oopoda against three bacterial strains Escherichia coli, Salmonella typhi and Staphylococcus aureus. Phytochemical assay confirmed the presence of terpenoid, flavonoids, saponins, tannins, phenolic compounds, carbohydrates, steroids and glycosides. Agar disc diffusion method was used to determine the zone of inhibition of the tested sample for antimicrobial activity. The crude methanolic extract showed activity against E. coli ZOI, 30.00±1.060 mm, for ethyl acetate fractions 50.00±4.18 mm, for chloroform fraction 27.00±0.060 mm and for n-hexane fraction 24.00±0.353 mm. This observation shows that ethyl acetate fraction possesses great potential against E. coli. Inhibition zone for Salmonella typhi was 23.25±1.050 mm for ethyl acetate, 14.00±0.353 mm for crude methanol extract, 22.00±1.753 mm for chloroform fraction and 08.00±0.352 mm for n-hexane fraction. This observation shows that n-hexane fraction possesses low potential against Salmonella typhi. Anti-bacterial potential against Staphylococcus aureus strain was maximum in ethyl acetate fraction and showed ZOI, 34.00±1.767 mm, for chloroform fraction 21.24±2.636 mm, for crude methanol extract 19.00±1.060 mm and for n-hexane fraction 16.00±1.412 mm respectively. Keywords: Antibacterial activity, Ferula oopoda; Phytochemical screening http://dx.doi.org/10.19045/bspab.2019.80016

Highlights

Introduction based on the IndoPakistan subcontinent it Infectious diseases are caused by was recorded that plant species were used pathogenic microorganisms; some for medication in Rigveda between 4500-organisms in extreme circumstances can1600 B.C
The Species Ferula oopoda belong to the family Apiaceae, subfamily Apioideae and genus Ferula.This plant is found in Balochistan region of Pakistan and found in neighbouring countries of Pakistan such as Iran, Afghanistan and India etc
Ferula oopoda plant was screened for its phytochemical constituent and antimicrobial activity

Summary

Introduction

Introduction based on the IndoPakistan subcontinent it Infectious diseases are caused by was recorded that plant species were used pathogenic microorganisms; some for medication in Rigveda between 4500-organisms in extreme circumstances can1600 B.C. Pakistan subcontinent it Infectious diseases are caused by was recorded that plant species were used pathogenic microorganisms; some for medication in Rigveda between 4500-. 1600 B.C. There is an extensive be fatal to the host. According to WHO 80 knowledge and research based. % of the world’s population uses plant applicability already exist in this region. Extracts or their active ingredients as folk. Some native plants are still widely used in medicine in several traditional treatments rural areas of Pakistan especially in [1]. Balochistan province due to their source of therapeutic agents. The research antimicrobial effect without any logical Layer between two different solvents appeared in separation funnel which was sealed

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Conclusion