Phytochemical profiling and screening of protective effects of Artabotrys odoratissimus on H2O2 induced oxidative stress in HEK-293 cells and erythrocytes

Abstract

ABSTRACT Artabotrys odoratissimus Blume commonly known as the ylang ylang vine is an aromatic woody shrub native to eastern Asia. The plant is used in traditional medicine systems; however, the bioactive potential of the plant remains unexplored. The present study focussed on the phytochemical profiling, anti-oxidative and cytoprotective effects of the stem bark ethanol extract (BEE) and fruit ethanol extract (FEE) on HEK-293 cells and erythrocytes. The preliminary group tests revealed the presence of alkaloids, phenols, flavonoids, lactones, glycosides, oils and fats. HR-LCMS analysis of bark ethanol extract revealed the presence of isocorydine, quercetrin, caffeic acid, juglone. In addition, GC-MS analysis of fruit ethanol extracts revealed the presence of furanone, pyrrole and long-chain methylated gamma-butyrolactone. BEE showed a total phenolic content of 573.30 ± 0.67 µg/mg and flavonoid content of 831.03 ± 1.61 µg/mg when compared to FEE which had total phenolic content and flavonoid content of 164.40 ± 0.24 µg/mg and 630.17 ± 2.79 µg/mg, respectively. Both the extracts showed protective effects on H2O2 induced oxidative damage on DNA, HEK-293 cells and erythrocytes. BEE showed an IC50 value of 2.23 ± 0.16 µg/ml in ABTS assay, similar results were observed in HEK-293 cells where the cells pre-treated with BEE showed a maximum cell viability of 56% under oxidative stress and the percentage inhibition of erythrocyte hemolysis was observed to be 86.39 ± 0.67% at a concentration of 250 µg/ml. The study clearly illustrates that the plant extracts from A. odoratissiumus possess potential antioxidative prospects against induced oxidative damage.