Abstract
Ethnopharmacological relevanceSickling of red blood cells (RBC) is known as the main phenomenon that initiates pathologic manifestations associated with several complications in sickle cell disease (SCD). In a previous work, the anti-sickling effect of the aqueous extract from the trunk bark and branches of Ceiba pentandra (L.) Gaertn (Malvaceae) has been demonstrated. Aim of the studyThe present study was conducted to identify the fraction with the most anti-sickle activity of the aqueous extract and subsequently to determine its main phytochemical groups. A bio-guided fractionation of this extract was carried out and a phytochemical screening of the most active fraction was performed. Materials and methodsBlood samples from SCD patients were treated with 2% sodium metabisulfite solution to induce hypoxia and sickling of erythrocytes; and then, incubated with each obtained fraction. The results showed that, among the three main fractions from the freeze-dried powder of the aqueous extract, the methanol insoluble fraction (IR) was the most active. This fraction was therefore subjected to solvent-solvent partitioning. ResultsThe hydrophilic sub-fraction (FA) from fraction IR showed the highest anti-sickling effect among all the sub-fractions. ConclusionThe phytochemical analysis revealed that catechin tannins, anthocyanosides, reducing compounds and amino acids were the main phytochemical groups of FA fraction.
Published Version
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