Abstract
In this present work, we described a bio-reduction method for the generation of silver nanoparticles (AgNPs) using aqueous leaf extract of Micrargeria wightii (M. wightii), which is a gifted alternative to other physicochemical routes. The prepared AgNPs were characterized by UV–visible spectroscopy (UV–vis), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray diffraction (X-RD), Transmission Electron Microscopy (TEM) with EDX and Dynamic light scattering (DLS). UV–visible spectrum showed a characteristic absorption peak at 440 nm of synthesized AgNPs. FT-IR analysis confirmed the existence of plant metabolites, which are responsible for the reduction of Ag (I) ions into Ag (0) NPs. X-RD pattern studies confirm the presence of the pure face-centered cubiccrystalline nature of Ag. Energy-dispersive X-ray (E-DX) spectrum showed the elemental composition of synthesized nanoparticles. Furthermore, TEM images confirm the formation of spherical shaped nano-silver particles with sizes ranging from 30 to 70 nm and supported by particle size analyzer, Dynamic Light Scattering (DLS). Thus, the present investigation provides an easy, eco-friendly and straightforward route for the synthesis of the antibacterial agent against Bacillus subtilis subtilis and Pseudomonas aeruginosa, with 15 and 13 mm zone of inhibition (ZOI) respectively.
Highlights
In the past few decades, nanotechnology is considered as one of the powerful and emerging technology with a lot of application in various fields such as agriculture, medical, textile, cosmetic and pharmaceutical research with an environmental impact [1, 2]
The prepared AgNPs were characterized by UV–visible spectroscopy (UV–vis), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray diffraction (X-RD), Transmission Electron Microscopy (TEM) with EDX and Dynamic light scattering (DLS)
The colloidal solution was examined with UV–Vis in the wavelength ranging from 300-600 nm for proximate analysis with is diluted to 1:10 (v:v) with the same solvent
Summary
In the past few decades, nanotechnology is considered as one of the powerful and emerging technology with a lot of application in various fields such as agriculture, medical, textile, cosmetic and pharmaceutical research with an environmental impact [1, 2]. The conventional method used for the synthesis of nanoparticles contains chemicals as both stabilizing and protecting agent, which was toxic in nature. These conventional methods can synthesis nanomaterial in a bulk amount with less time. In order to overcome the limitation and demerits of the conventional method, green synthesis can be act as an alternative method [7] It is considered as one of the prominent areas in the field of bionanotechnology, which provides benefits to both economy and environment and it does not produce any by-products and pollution. Bacillus subtilis has been implicated in several cases of food poisoning This present work describes a bio-reduction method for generation of AgNPs using aqueous leaf extract of M. wightii. The obtained extract solution was stored at 4 °C for the preparation of AgNPs
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