Phytochemical investigation and evaluation of antimicrobial activity of different extracts of Swietenia macrophylla barks

Abstract

Event Abstract Back to Event Phytochemical investigation and evaluation of antimicrobial activity of different extracts of Swietenia macrophylla barks Velaga V. Appalaraju1, 2*, Sheryar Afzal2 and Fadhiratul Hashmin Jafry2 1 Mahsa University, Malaysia 2 Faculty of Pharmacy, Mahsa University, Malaysia Background The plant Swietenia macrophylla possesses therapeutic uses like antibacterial, antifungal, antidiarrhoeal, anti-inflammatory, hypoglycaemic, antioxidant, cytotoxic, antimalarial and antidiabetic activities. The purpose of this study is to determine the phytochemical composition and antimicrobial activity of two different extracts of S. macrophylla bark against six bacterial strains. Methods Swietenia macrophylla bark powder was extracted by Soxhlet extraction method by using acetone and methanol. The extract was characterised by Shinoda test, Fehling’s test, Wagner’s test and Biuret test. Different concentration (10 and 20 mg/mL) of plant extracts were tested by agar well diffusion method by using bacterial strains Bacillus subtilis, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Klebsiella pneumoniae and Proteus vulgaris and compared with tetracycline (0.05 mg/mL). Results Extracts of S. macrophylla barks revealed the presence of flavonoids, alkaloids, phenolic compounds, carbohydrates, proteins, fats and oils. The most susceptible bacterial strains were P. aeruginosa and S. aureus for methanol extract and acetone extract respectively. Antibacterial screening with acetone and methanol extracts showed both significant (p<0.05) and not significant (p>0.05) zone of inhibition. The MIC was found in the range between 0.63 to 2.50 mg/mL. Conclusion Swietenia macrophylla bark showed that it has broad spectrum of antimicrobial activity that appear as potential alternative therapeutic agent against certain microorganisms in future drug development. Acknowledgements The authors would like to thank the management of MAHSA University for funding this project. Keywords: Swietenia macrophylla, Antimicrobial activity, Agar well diffusion, Zone of inhibition, minimum inhibitory concentration Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Inflammatory diseases Citation: Appalaraju VV, Afzal S and Jafry F (2019). Phytochemical investigation and evaluation of antimicrobial activity of different extracts of Swietenia macrophylla barks. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00098 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 24 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Velaga V Appalaraju, Mahsa University, Kuala Lumpur, Malaysia, velagaappalaraju@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Velaga V Appalaraju Sheryar Afzal Fadhiratul Hashmin Jafry Google Velaga V Appalaraju Sheryar Afzal Fadhiratul Hashmin Jafry Google Scholar Velaga V Appalaraju Sheryar Afzal Fadhiratul Hashmin Jafry PubMed Velaga V Appalaraju Sheryar Afzal Fadhiratul Hashmin Jafry Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.