Abstract
Abstract The chemical constituents and antioxidant activity of essential oil from Rumex nepalensis growing in Himalayan region was evaluated. The essential oil was obtained from steam distillation of fresh plant and analyzed by Gas Chromatography and Gas Chromatography-Mass Spectrometry in relation with their Kovat retention indices and mass spectra. The essential oil was rich in monoterpenoids and oxygenated monoterpenoids. The major chemical constituents were identified from roots of Rumex nepalensis as hemellitol (10.80%), 1,1,3-trimethyl-cyclohexane (9.17%), n-nonane, (7.05%), linalool oxide (3.87%), 4-methyl-octane (3.58%), 14-oxy-α-muurolene (3.56%), α-humulene (3.53%), khusinol (3.21%), 2,6-ditertary-butyl-phenol (3.09%), tridecene (2.94%), E-caryophyllene (2.83%), α-copaene (2.36%), δ-cadinene (2.32%), ethyl-cyclohexane (2.18%) & 3,5-dimethylheptane (2.09%) where as oxygenated sesquiterpenoids were minor constituents. Our study showed there is little known about the chemical composition of essential oil from Rumex nepalensis along with other minor constituents. The free radical scavenging property of Rumex nepalensis employed by in-vitro assay methods like scavenging activity of 2,2-diphenyl1-picrylhydrazyl (DPPH) and scavenging of H2O2 activity was 83.10% & 79.18% at 600 µg/mL concentrations, respectively. In this study, the mean chemical constituents of roots of Rumex nepalensis was reported first time from roots which may contribute to its higher antioxidant activities and provide scientific evidences for its traditional uses.
Published Version
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