Abstract
Curcuma amada Roxb. is a valuable medicinal herb with food, economic, pharmaceutical, and industrial value. This plant is commonly propagated through rhizome which is season dependent and slow process. Moreover, rhizomes are easily infected by different soil-borne pathogen, which deteriorates the quality and quantity of production. Hence, this study was carried out to develop one step plant regeneration protocol using rhizomatic bud on meta-Topolin (mT) supplemented medium. About 600 plants were produced on Murashige and Skoog (1962) (MS) medium added with 3.0 mg/L mT after twelve weeks of culture. Well rooted plants were successfully hardened (95%) and subsequently all the plants were established in the field. Quantitative phytochemical analysis and assessment of chemical composition of essential oil by Gas Chromatography - Mass Spectroscopy (GC-MS) confirmed the biochemical fidelity of the field-established tissue culture plants. Robust therapeutic activities such as antioxidant, anticancer, and antimicrobial activities were found in the rhizome extract and essential oil of the field-established tissue culture plants. It was concluded that tissue cultured plants are at par with the mother plant in terms of phytochemicals composition and therapeutic activity. Thus, this protocol could be useful for the production of a large number of plants aiming for its conservation, propagation as well as sustainable utilization in food, beverage, and pharmaceutical industries.
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