Abstract
Aim of the present study was to determine theIn-vitroantibacterial activity of ethanolic extract of E. globulus leaves against common multidrug resistant poultry pathogens. Phytochemical analysis through HPLC revealed that kaempeferol (7.315min) followed by querecetin (6.655min) and myrecetin (3.655min). Percent area of kaempeferol (6826.88%) was highest, followed by myrecetin (5516.22%) and querecetin (163.748%). Phytochemical investigation of ethanolic extract ofE. globulusleaves through GCMS showed highest retention time (min) α-pinene (20.43) and α-terpineol (20.15) accompanied by spathulenol (11.97), piperitone (11.04). The ethanolic extracts ofE. globulusleaves showed a highest zone of inhibition againstS. pullorumSP6; 20.64± 2.08, E. coli SE 12; 19.75± 2.83, C. perfringenstype A (CPM38-01); 19.46± 2.02. The highest level of MIC of E. globulus noted were against S. gallinarumS22; 133.37±53.294, S. gallinarumS1; 130.20±45.10, S. gallinarumS4; 129.47±24.182, S. gallinarumS3; 126.83±72.392. In conclusion, the study confirmed that the ethanolic extract ofE. globulusis composed of active ingredients having antibacterial activity and can be referred as an alternate to antibiotics.
Highlights
The poultry sector retains a valuable contribution to the agriculture sector and Pakistan’s economy [1]
Antimicrobial testing of ethanolic extract of E. globulus leaves against E. coli, S. pullorum, S. gallinarum and C. perfringens type A was done through Agar well diffusion and Minimum inhibitory concentration
The ethanolic extract obtained from the E. globulus leaves was screened for various phytochemicals
Summary
The poultry sector retains a valuable contribution to the agriculture sector and Pakistan’s economy [1]. Antibiotic resistance issues in both animal and human treatment, the researchers are in search to find an alternative to antibiotics used in poultry feed. It may curtail a number of antibiotics entering into the individual food chain and food industries [6]. Research studies have shown that plant extract has antibacterial activity and endeavors against other pathogens. The present study aimed to investigate the phytochemicals composition and antimicrobial activity of E. globulus against poultry pathogens such as E. coli, S. pullorum, C. perfringens type A and S. gallinarum
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