Abstract
BackgroundLathyrus sativus L. (Fabaceae) has long been used as a traditional medicine for the treatment of several ailments such as Scabies, eczema, and allergy. The aim of the study was to evaluate the phytochemical nature with Central Nervous System (CNS) depressant, analgesic, antipyretic activities of the methanolic plant extract of Lathyrus sativus L. seeds in different experimental models.MethodsPreliminary phytochemical screening and proximate analysis was carried out using different standard methods. CNS depressant activity was evaluated observing the effects of plant extract on Swiss albino mice using open field and hole-cross method. Acetic acid induced writhing and formalin induced paw licking methods were used for the appraisal of analgesic activity while 2,4-dinitrophenol (DNP) induced pyrexia model was used to investigate the antipyretic activity. The data were analyzed by one way ANOVA followed by Dunnett’s test using SPSS (version 20).ResultsThe phytochemical analysis revealed the presence of wide range of phytoconstituents in the plant extract. Our investigation demonstrated that the methanolic plant extract significantly (p < 0.001) decreased the locomotor activity of mice in open field and hole-cross method at both the tested doses (200 and 300 mg/kg) which were comparable to the standard drug diazepam (1 mg/kg). The plant extracts significantly (p < 0.001) inhibited the writhing induced by acetic acid in mice to 87.09% and 80.65% (200 and 300 mg/kg respectively) compared to the standard indomethacin (70.97%). The extracts (200 and 300 mg/kg respectively) also significantly (p < 0.001) reduced the writhing to 43.39%, 64.15% in early and 46.15%, 97.44% in late phase of formalin-induced licking and biting. In 2,4-DNP induced pyrexia the extracts exhibited protection at 200 and 400 mg/kg, similar to standard drug aspirin at 150 mg/kg.ConclusionThe results demonstrated that the plant extract has potential CNS depressant, analgesic and antipyretic activity.
Highlights
Lathyrus sativus L. (Fabaceae) has long been used as a traditional medicine for the treatment of several ailments such as Scabies, eczema, and allergy
Central Nervous System (CNS) depressant activity Open field test After statistical analysis of the experimental data (Dunnett’s test), it was observed that in open field test, the number of squares traveled by the mice was suppressed significantly in the test group throughout the study period (Table 3)
The CNS depressant activity observed for the extract was dose dependent and a noticeable result was found at 120 min of test sample administration
Summary
Lathyrus sativus L. (Fabaceae) has long been used as a traditional medicine for the treatment of several ailments such as Scabies, eczema, and allergy. The aim of the study was to evaluate the phytochemical nature with Central Nervous System (CNS) depressant, analgesic, antipyretic activities of the methanolic plant extract of Lathyrus sativus L. seeds in different experimental models. Medicinal plants have provided us lots of bioactive natural compounds like alkaloids, carbohydrates, glycosides, saponins, flavonoids, Depression of central nervous system (CNS) can be considered as a major affective brain disorder which is very prevalent now-a-days as about 5% of the general population is found to be suffering from it [4]. Researchers are focusing towards traditional complementary and alternative medicines to discover new drugs for the treatment of psychiatric disorders, alleviating pain and fever [9,10,11]
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