Phytochemical Analysis of Terpenes from Eucalyptus camaldulensis and Study of its Biological Effect Against Macrophomina spp.

Abstract

This research was carried out under laboratory conditions to test the inhibitory ability of Eucalyptus camaldulensis leaf extracts (alcoholic and oily) on the pathogenic fungus Macrophomina phaseolina, which was isolated from strawberry roots and diagnosed based on its morphological and microscopic characteristics. The active compounds present in the volatile oil separated from the leaves of E. camaldulensis were identified by using (GC-MS). And obtaining the effective compounds (Gamma-Terpinene, Benzene, Terpinen-4-ol, carvone, 1-Cyclohexene, p-Cymen-7-ol, 2-Naphthalene methanol, 5-methanol, Methyl1-4methylene). The separated fatty acids were identified from the ether petroleum extract of eucalyptus leaves using GC-MS and obtaining fatty acids (Lumaric acid, Linolic acid, Pentadecanoic acid, Hexadecanoic, a-Lenolinic, Stearic acid, Eicosenoic acid, Arachidonic acid Palmatic acid, Oleic, Octadecanoic acid). The Inhibition effect of eucalyptus leaf extract (alcoholic and oily) against the fungus Macrophomina phaseolina was tested. The results showed that the petroleum ether and eucalyptus oil extracts that were used at concentrations of 5, 10 and 15 mg/ml in the PDA medium had inhibitory effects on the growth of the aforementioned fungus. Both extracts caused a significant effect on the growth of this fungus in All concentrations used, as these fungi failed to form mycelium on PDA medium.