Phytochemical Analysis of Robinia Pseudoacacia Flowers and Leaf: Quantitative Analysis of Natural Compounds and Molecular Docking

Abstract

Phenolic compounds are widely found and well-known secondary metabolites in plants. Identification, quantification of phenolic compounds and determination of their biological activities reveal the unknown secrets of plants. Robinia pseudoacacia (RP) is known as the white-flowered false acacia and is distributed in Northern Anatolia in Turkey. Spectrophotometric and chromatographic techniques are used to identify the presence and amount of phenolics. In this study, RP flowers and leaf was extracted with methanol and analyzed by LC-MS/MS to determine its phytochemical content. Salicylic acid and syringic acid were found as major products in leaves and flowers. RP extracts have been reported to have antibacterial activity and BChE inhibitory properties. Therefore, the BChE and DD peptidase enzyme inhibitory properties of main components salicylic acid and syringic acid were investigated by moleculer docking (MolDock). According to MolDock results, syringic acid interacted with BChE and DD peptidase were calculated as a MolDock score -79.38, -71.25, binding energies -5.90, -5.40 kcal/mol respectively. Salicylic acid interacted with BChE and DD peptidase were calculated as a MolDock score -63.54, -66.18, binding energies -6.10, -5.70 kcal/mol respectively. As a result, Salicylic acid had higher binding energy in its interactions with BChE and DD peptidase enzymes. In theory, salicylic acid can be used as a good BChE and DD peptidase inhibitor.