Abstract
AbstractCystic Echinococossis is a serious zoonotic parasitic infection caused by Echinococcus granulosus species complex. The current study was designed to evaluate the in-vitro antiprotoscolices effect of alcoholic extracts of three selected medicinal plants including Buxus Wallichiana, Berberis vulgaris and Euphorbia heliscopia against Echinococcus granulosus. Fertile hydatid cysts were collected from livestock and viability of the protoscolices was confirmed by 0.1% eosin red stain method. Protoscolices were subjected to three different concentrations of alcoholic extracts (10mg/ml, 30mg/ ml and 50mg/ml) for 10, 20 and 30 min. The highest efficacy was shown by B. vulgaris (97.92%) followed by B. wallichiana (65.98%) and E. heliscopia (61.22%) respectively, after exposure of 30 minutes at 50mg/ml concentration, that lead to the significant reduction in the viability of protoscolices. Alkaloids, flavonoids, tannins and saponnins were identified qualitatively and weighted quantitatively, that might help in the identification of bioactive compounds involved in selective action on the tegument layer of protoscolices. Alcoholic extracts of all the three selected medicinal plants showed toxic activities against protoscolices of Echinococcus granulosus. These findings suggest that all the selected medicinal plants could be a promising source of potent antiprotoscolices effect. However, the mechanism by which plant extracts killed protoscolices and also their safety for living cells are unclear and need to be investigated further.
Highlights
Cystic Echinococcosis (CE) or Hydatidosis is a zoonotic parasitic disease, caused by the larval stage of small tapeworm of dog of genus Echinococcus
The Voucher specimens of B. wallichiana (BW1), B. vulgaris (BV-1) and E. heliscopia (EH-1) of the plants are deposited in the local herbarium of Abdul Wali Khan University Mardan Pakistan
The fertility and reproducibility of the hydatid cysts is determined by the presence of free protoscolices in wet mount of hydatid cyst fluid
Summary
Cystic Echinococcosis (CE) or Hydatidosis is a zoonotic parasitic disease, caused by the larval stage of small tapeworm of dog of genus Echinococcus. CE causes extensive mortality and morbidity in human and livestock [1]. As a result of CE, Hydatid cysts developed in internal organs mainly in liver and lungs of intermediate hosts and in humans as unilocular fluid filled bladder [3]. This infection remains asymptomatic initially for many years or even permanently [4]. The use of efficient protoscolicidal agent is necessary in order to reduce the risks of intraoperative spillage of the cysts fillings during
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