Phytochemical Analysis and Repellent Activity of Annona Muricata and Syzygium Samarangense Leaves Against Sitophilus Oryzae

Abstract

The present study is conducted to control the stored pest grain - Sitophilus oryzae using plant leaves, such as Annona muricata and Syzygium samarangense. Extraction of selected leaves were done using organic solvents such as Methanol and petroleum ether. Qualitative phytochemical analysis of selected plant leaves revealed the presence of secondary metabolites such as Alkaloids, Flavonoids, Tannins, Saponins, Glycerides, Steroids, Terpenoids, Phenols, Quinone and Phlobotannine, which have repellent activities against Sitophilus oryzae. Repellency test were also done using Methanol and petroleum ether leaf extract Annona muricata and Syzygium samarangense. No repellent activity were observed in Petroleum ether leaf extract of Annona muricata in all concentration. Methanol leaf extract of Annona muricata showed highest repellency of 61.33% at 6.4% concentration in 24 hrs. Petroleum ether leaf extract of Syzygium samarangense showed highest repellency of 55.00% at 6.4% concentration in 24 hrs, where on methanol leaf extract of Syzygium samarangense showed highest repellency of 60.00% at 6.4%. These leaf extracts can be used as an alternative biopesticides instead of chemical pesticides, which would be safer for storing rice grains.