Phytochemical analysis and Invitro antioxidant activity of silver nanoparticle synthesized Crateva religiosa bark extract

Abstract

The aim of the study is to determine the phytochemical analysis and antioxidant activity of silver nanoparticle synthesized aqueous bark extract of Crateva religiosa. The aqueous extract of Crateva religiosa barks are prepared by maceration method. The qualitative phytochemical and quantitative phytochemical analysis was carried out using standard procedures. Silver nanoparticle was synthesized for the aqueous extract. Then the antioxidant activity was determined by DPPH scavenging activity and FRAP assay. The qualitative phytochemical analysis shows the presence of alkaloids, flavonoids, phenols, tannins, saponins. Total phenol content of bark extract was found to be 1.423mg/g. Total flavonoid content was found to be 1.6mg/g.UV spectrophotometry peak confirms the presence of silver nanoparticle. The extract shows effective antioxidant property with IC50 value 42.32 µg/ml for DPPH assay and IC50 value 45 µg/ml for FRAP. The Crateva religiosa bark extract could be a novel source of antioxidant.