Abstract
The present study examined the antioxidant effect of phytic acid on iron (II)-enhanced hydroxyl radical (•OH) generation induced by 1-methyl-4-phenylpyridinium ion (MPP +) in the extracellular fluid of rat striatum. Rats were anesthetized, and sodium salicylate in Ringer’s solution (0.5 nmol/μl/min) was infused through a microdialysis probe to detect the generation of •OH as reflected by the non-enzymatic formation of 2,3-dihydroxybenzoic acid (DHBA) in the striatum. Phytic acid (100 μM) did not significantly decrease the levels of MPP +-induced •OH formation trapped as 2,3-DHBA. To confirm the generation of •OH by the Fenton-type reaction, iron (II) was infused through a microdialysis probe. Introduction of iron (II) (10 μM) enhanced MPP + induced •OH generation. However, phytic acid significantly suppressed iron (II)-enhanced •OH formation after MPP + treatment ( n=6, P<0.05). These results suggest that the antiradical effect of phytic acid occurs by chelating iron required for the MPP +-enhanced •OH generation via the Fenton-type reaction.
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