Abstract
The effect of nutrient media, temperature, pH level, carbon and nitrogen ion concentration were studied on mycelial growth and sporulation of Alternaria porri caused purple blotch of onion. The investigations revealed that Potato dextrose agar was the best culture medium for A. porri. The maximum mycelial growth of A. porri was recorded on 30°C temperature (85.74 mm) and pH 7.0 (83.40 mm). A. porri grew significantly better response to the source of carbon nutrient media on mycelial growth and observed the maximum mycelial growth on maltose (88.26 mm) based medium with highest sporulation and potassium nitrate-based media source of nitrogen gave maximum mycelial growth (80.75 mm) and sporulation of A. porri. The present findings are useful for the preparation of inoculums required for resistance breeding and fungicidal evaluation against pathogen A. porri.
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