Abstract
IN A STUDY of the anterior pituitary of the female rabbit (1, 2) it was found that striking changes occurred in the composition of the acidophil cells after mating. This reaction was demonstrated by means of a method which separated the acidophils into two distinct tinctorial types (3). The glands were fixed in sublimateformalin followed by an adaptation of Heidenhain's azan modification of Mallory's connective tissue stain. The ordinary or standard acidophil was colored yellow with orange G. Other acidophil cells which become conspicuous during sexual activity were characterized by the coarseness and refractivity of their granules, and took a brilliant red stain due to their special affinity for azocarmine. The chromophobes were not colored or appeared a light pink, and the basophils stained blue with analinblue. In estrous rabbits the modified acidophils (carmine cells) are usually small and inconspicuous, but after mating they increase in size and number and become
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