Abstract
P OTATO VIRUS Y (PVY) is one of the most destructive aphid transmitted pathogen to potato plants worldwide. In Egypt, PVY infection causing about 80% reduction the global yield of potato. The objective of this study was to characterize Potato virus Y (PVY-EG) infecting potato plants, based on biological, serological and molecular properties. Naturally infected potato plants by PVY gave positive reaction with PVY-polyclonal antibodies using DAS-ELISA for virus identification. PVY has a higher stability at 45 h (Longevity), 66°C at 10 min (TIP) at 10-6 (DEP), revealing presence of cytoplasmic and crystalline inclusion bodies of the epidermal strips from infected Datura metel (diagnostic host) leaves at 12 days post PVY inoculation. The PVY has UV-spectra at λ 235 and at λ 257 nm. PVY yield was 1.25/100 g leaf tissues and 260/280 more than 1 (it was 1.6). The viral particles were rod flexible (helical symmetry) of 11 x 570 nm, with obvious immunogenicity that represented by 1:1024 titer of antibodies. The cDNA fragment of CP gene was 610 bp. Sequence analysis revealed that PVY isolate showed 93-99% similarity with other worldwide PVY isolates.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
