Abstract
The present study investigated the relationships between egg viability and ovarian fluid composition, egg physiology and egg metabolism in lake trout, Salmo trutta lacustris, to obtain biomarkers for egg quality determination. The ovarian fluid pH, protein levels and activities of aspartate aminotransferase and β-d-glucuronidase were significantly correlated with egg viability expressed as the number of eyed stage embryos. Regression models demonstrated that an ovarian fluid pH between 8.44 and 8.57, protein levels below 235.56 mg 100 ml−1ovarian fluid, aspartate aminotransferase activity below 31.65 μm min−1 l−1ovarian fluid and β-d-glucuronidase activity below 8.62 μm min−1 l−1 ovarian fluid characterized egg batches with high viability (≥80%).
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