Relationship between eyed rate and the biochemical composition of eggs and ovarian fluid in Hucho taimen

Abstract

We investigated the relationship between the eyed rate and the biochemical composition of eggs and ovarian fluid in Hucho taimen.We measured enzyme activity and levels of protein,amino acids,vitamins,and minerals in stage V eggs and ovarian fluid.We used each of these parameters as an independent variable in a re-gression model to predict the eyed rate.There was a significant correlation between the eyed rate and Mg2+-AT-Pase activity,phosphorus levels,and amino acids in the eggs.There was no correlation between the eyed rate and the remaining parameters(protein,acid phosphatase,alkaline phosphatase,aspartate aminotransferase,succinate dehydrogenase,calcium ions,iron ions,vitamin C,and vitamin E).Egg batches with high viability(≥80%) were characterized by the following: Mg2+-ATPase activity above 8.3×10-5 μmolPi/(min·mgprot),levels of phosphorus between 12.97 and 23.03 μmol/g egg,and levels of amino acids between 574.89 and 1 195.40 μmol/g egg.In con-trast,in the ovarian fluid only aspartate aminotransferase activity was correlated with the eyed rate of H.taimen.Egg batches with high viability(≥80%) had aspartate aminotransferase activity 18.14 μmol/min/L ovarian fluid.