Abstract
Abstract Traditional cell culture media such as RPMI is consistently used in in-vitro studies in order to inform in-vivo translation. However traditional media poorly simulates physiological conditions which can lead to inaccurate interpretations of data and failed in-vivo applications. The recent development of human plasma like media (HPLM) mirrors physiologic conditions with more realistic levels of glucose, amino acids and other nutrients. We investigated bone marrow derived macrophage phenotype and activation in either RPMI or HPLM to better understand the effects of physiologic conditions on macrophages behavior. Classically activated M1 (100ng/mL LPS + 10ng/mL IFNγ) or alternatively activated M2 (20ng/mL IL-4) were investigated for metabolic activity and behavior. Macrophages cultured in HPLM exhibit a unique phenotype compared to RPMI with increased iNOS expression in response to LPS treatment and reduced CD206 after treatment with IL-4. These data suggest physiologic media can alter the behavior and function of macrophages in-vitro. Following more investigation, these unique activation states in physiologic conditions may uncover differential metabolic requirements and behavior not previously seen in traditional culture media.
Published Version
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