Abstract
Kefiran is a hydro-soluble polysaccharide, which can be isolated from kefir grains biomass. In the present study was characterised physicochemical and morphological properties of kefiran isolated from kefir grains biomass, obtaining from a local dairy company. High performance liquid chromatography analysis of monosaccharides revealed that kefiran was composed of glucose and galactose in an approximate ratio of 1.0:0.4 in nature. Infrared spectra of kefiran revealed the presence of carboxyl, hydroxyl, and amide groups, which corresponded to a typical heteropolymeric polysaccharide, which indicated a purified structure of isolated kefiran from kefir grains biomass.
Highlights
Kefiran, a hydro-soluble prebiotic branched exopolysaccharide, is a hydroxyl groups rich, and heteropolysaccharide biopolymer containing glucose and galactose [1,2,3,4]
Kefiran is produced either from kefir grains or pure cultures kefiran of Lactobacillus kefiranofaciens under aerobic conditions or mixed cultures of Lactobacillus kefiranofaciens with Saccharomyces cerevisiae under anaerobic conditions [6,7,8,9]
According to method reported by Adıgüzel and Tunçer, [22] sugar composition of kefiran EPS carried out by TLC that showed the presence of glucose and galactose and was in agreement with previous results [19,27]
Summary
A hydro-soluble prebiotic branched exopolysaccharide, is a hydroxyl groups rich, and heteropolysaccharide biopolymer containing glucose and galactose [1,2,3,4]. According to chemical structure of kefiran, it is a branched hexa- or hepta-saccharide repeating units, that itself is composed of a regular pentasaccharide unit to which one or two sugar residues are randomly attached. It had a backbone composed of (1 → 6)-linked Glc, (1 → 3)-linked Gal, (1 → 4)-linked Gal, (1 → 4)-linked Glc and (1 → 2, 6)-linked Gal, with branch attached to O-2 of Gal residues and Glc residues situated in the structure end [2]. Kefiran is produced either from kefir grains or pure cultures kefiran of Lactobacillus kefiranofaciens under aerobic conditions or mixed cultures of Lactobacillus kefiranofaciens with Saccharomyces cerevisiae under anaerobic conditions [6,7,8,9]
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