Physical Methods on Biological Membranes and Their Model Systems

Abstract

Physical methods on biological membranes and their model system: A perspective.- High resolution NMR spectroscopy in liquids and solids.- Basic principles of deuterium and phosphorus-31-magnetic resonance.- Use of electron spin resonance to study complex biological membranes.- Freeze-etching electron microscopy: recent developments and application to the study of biological membranes and their components.- The nature of intramembraneous particles.- Lateral and polymorphic phase transitions in relation to the barrier function of a lipid membrane.- Fluorescence energy transfer as a structural probe in membranes and membrane in bound proteins.- The interaction of intrinsic proteins and lipids in biomembranes.- Conformational studies of membrane proteins by high resolution NMR.- Water organization in reversed micelles.- Lipid-protein interactions: from crystalline lipoproteins to intact membranes.- Membrane fusion and lipid polymorphism.- Control of membrane fusion by divalent cations, phospholipid head-groups and proteins.- Correlation between steady-state and time-resolved fluorescence anisotropy data.- Membrane lipid fluidity at a physiologically relevant scale: determination by fluorescence polarization.- Dynamic interaction of fluorescently labeled adenosine deaminase in intact human erythrocytes: relation to abnormal activity in malignant cells.- Phase separation in phospholipid mixtures by fluorescence anisotropy.- Membrane fluidity and lipid composition.- Membrane fluidity and membrane activities.- Membrane fluorescence anisotropy behaviour during cell cycle..- Stable biomembrane surfaces formed by phospholipids polymers.- Utilizing mast cells and basophils to study lipoxygenase involvement in membrane activation.- Immunoglobulin surface adsorption studied by total internal reflection with fluorescence correlation spectroscopy.- Enthalpy state of different liposome preparations of dimyristoyl phosphatidylcholine and their stability in the presence of a complex forming protein.- Free-flow electrophoresis as a suitable tool to explore intervesicular interactions.- Solid Atate 19F NMR in "Model" membrane systems.- Organized cultures of nerve tissues: a novel model system for studies of lipid protein interaction on the functional level.- Partition of proteins between water and non-polar phases.- Photomodification of Biomembranes.