Abstract
The effect of lipid peroxidation on the lipid fluidity of porcine intestinal brush-border membranes was examined by measuring the rotational mobility and the accessibility to fluorescence quenchers (CH 3COOTI, CuSO 4 and KI) of pyrene or n-(9- anthroyloxy) stearic acid ( n = 2 or 12) in the membranes. The harmonic mean of the rotational relaxation times of pyrene increased and the rate constants, k q , of the quenching reaction of pyrene and 2-(9-anthroyloxy)stearic acid incorporated in the membrane lipids decreased upon lipid peroxidation, indicating reduction of the lipid fluidity of the membranes by lipid peroxidation. In addition, the k q value of the reaction of 2-(9-anthroyloxy)stearic acid in the membranes with Cu 2+ decreased in proportion to the amount of the products of lipid peroxidation. On the other hand, the k q value of the reaction of 12-(9-anthroyloxy)stearic acid with Cu 2+ or I − was unaffected by lipid peroxidation. Based on these results, a localized change in the lipid fluidity of the membranes in association with lipid peroxidation has been discussed.
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