Phylogroup classification and investigation the relationships between phylogroups and antibiotic resistance patterns of uropathogenic E. coli isolated from pediatric urinary tract infection

Abstract

Background & objectivesOne of the most challenging therapeutic fields in Pediatrics is treating urinary tract infection (UTI) caused by the most common pathogen, uropathogenic Escherichia coli (UPEC), with a different distribution of phylogenetic groups that acquired a variety of antibiotic resistance phenotypes. The aims of this study were to determine the prevalence of phylogroups and find out the relationship between resistance induced and phylogroup dominance among children with UTI in Hamadan, Iran. MethodsIn this cross-sectional study, 140 uropathogenic E. coli isolates from children with UTI were investigated. Antimicrobial susceptibility testing against 6 antibiotic classes was performed by the disk diffusion method. Isolates were subjected to phylogenetic typing using the quadruplex polymerase chain reaction (PCR) method. ResultsAntibiogram results indicate that the highest and lowest resistance were against co-trimoxazole (64.3%) and meropenem and imipenem (0%), respectively. Phylogroup B2 (27.9%) was predominant followed by E (26.4%), A (16.4%), Clade1 (10.7%), B1 (5.7%), C (5.0%), F (4.3%), D (2.9%) and unknown (0.7%) phylogroups. 42.1% of E. coli bacteria were multi-drug resistant (MDR) with most belonging to phylogroups E (30.5%) and B2 (27.1%). ConclusionBesides phylogroup B2, phylogroup E is also dominant and a cause of much antibiotic resistance in our study population.