Phylogeography reveals the origin of the two phenological forms of large blue, Phengaris arion (Lepidoptera: Lycaenidae)

Abstract

Abstract Our main goal was to investigate the phylogeography of the butterfly Phengaris arion to reveal the evolutionary origin of its ‘spring’ and ‘summer’ forms. Molecular analyses based on highly variable microsatellites, together with Wolbachia screening, were carried out on 34 populations in Europe. We found three well-defined genetic lineages of different origins: the Apennine, the central and the eastern. The highly distinct Apennine lineage is limited by the Alps and evaluated as an Evolutionary Significant Unit (ESU). Therefore, the taxon name ligurica, described from the Ligurian coast (Italy), should not be applied to denote the ‘summer form’ of the Pannonian region. The central lineage is limited by the Carpathians and the most eastern ranges of the Alps, and lacks major range fluctuations related to glaciations, although there is evidence for extra-Mediterranean refugia in the Carpathian Basin. The eastern clade could have had refugia in central Asia. Our results exclude the potential allopatric origin of the ‘spring’ and ‘summer’ arion, and support the hypothesis that the existence of the two forms could be a result of local adaptation to the distinctive phenology of host plant flowering which is manifested in the genetic differences between them. Wolbachia infection has been ruled out as a driver of sympatric speciation in P. arion.