Phylogeny and Density Dynamics of Wolbachia Infection of the Health Pest Paederus fuscipes Curtis (Coleoptera: Staphylinidae).

Ary A Hoffmann,Jiayao Hu,Jianqing Zhu,Jie Fang,Weidong Yu,Zimiao Zhao,Chen Ge,Shuojia Ma,Weibin Jiang,Li Shen

doi:10.3390/insects11090625

Ary A Hoffmann, Jiayao Hu + Show 8 more

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https://doi.org/10.3390/insects11090625

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Journal: Insects	Publication Date: Sep 11, 2020
Citations: 8	License type: CC BY 4.0

Affiliation: University of Melbourne, Shanghai Normal University

Abstract

Simple SummaryWolbachia pipientis is a maternally inherited endosymbiont of arthropods and filarial nematodes, and was reported to occur in Paederus fuscipes, a beetle that causes dermatitis linearis and conjunctivitis in humans when they come in contact with skin. In this study, we report the phylogenetic position and density dynamics of Wolbachia in P. fuscipes. The phylogeny of Wolbachia, based on an analysis of MLST genotyping, showed that Wolbachia from P. fuscipes belongs to supergroup B. Quantitative PCR indicated that the infection density in adults was higher than in any other life stage (egg, larva or pupa), and that reproductive tissue in adults had the highest infection densities, with similar densities in the sexes. These findings provide a starting point for understanding Wolbachia infection dynamics in P. fuscipes, and interactions with other components of the microbiota.The maternally inherited obligate intracellular bacteria Wolbachia infects the reproductive tissues of a wide range of arthropods and affects host reproduction. Wolbachia is a credible biocontrol agent for reducing the impact of diseases associated with arthropod vectors. Paederus fuscipes is a small staphylinid beetle that causes dermatitis linearis and conjunctivitis in humans when they come into contact with skin. Wolbachia occur in this beetle, but their relatedness to other Wolbachia, their infection dynamics, and their potential host effects remain unknown. In this study, we report the phylogenetic position and density dynamics of Wolbachia in P. fuscipes. The phylogeny of Wolbachia based on an analysis of MLST genotyping showed that the bacteria from P. fuscipes belong to supergroup B. Quantitative PCR indicated that the infection density in adults was higher than in any other life stage (egg, larva or pupa), and that reproductive tissue in adults had the highest infection densities, with similar densities in the sexes. These findings provide a starting point for understanding the Wolbachia infection dynamics in P. fuscipes, and interactions with other components of the microbiota.

Highlights

Wolbachia pipientis is the most widespread endosymbiotic bacterium of insects and other arthropods, infecting perhaps two-thirds of present-day insect species, as well as about 40% of terrestrial arthropod species [1]
The transmission of Wolbachia is predominantly vertical and secondarily horizontal [2]. It can induce a number of reproductive manipulations in its host, including cytoplasmic incompatibility [3], thelytokous parthenogenesis [4], feminization of genetic males [5] and male killing [6]
The strain identified by the Wolbachia MLST database has the designation

Summary

Introduction

Wolbachia pipientis is the most widespread endosymbiotic bacterium of insects and other arthropods, infecting perhaps two-thirds of present-day insect species, as well as about 40% of terrestrial arthropod species [1]. Wolbachia may generate positive fitness effects on numerous hosts, such as filarial nematodes, fruit flies, bedbugs and wasps [7,8,9,10], and decrease host transmission of dengue [11], malaria [12], West Nile virus [13] and other pathogens [14]. It is considered as a novel method for controlling mosquito- and vector-borne human diseases [15]. Population replacement approaches involve the release of both male and female mosquitoes that carry a heritable factor that reduces or blocks their ability to transmit viruses [15]

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