Abstract
Acacia species are found at various arid and semiarid regions. Among the tree genera of family, Fabaceae, Acacia contains the highest number of species. We have collected different species of Acacia from various places of Saudi Arabia and reconstructed phylogeny for evaluation of genetic relationship among them. Internal transcribed spacer sequence of nrDNA (nrDNA-ITS) locus was used for the reconstruction of phylogeny among these species. Based on phylogenetic tree, Acacia etbaica and A. johnwoodii were close to each other. Similarly, A. ehrenbergiana and A. tortilis were close to each other. Thus, this gene locus was helpful in evaluating the genetic relationship among these species.
Highlights
Trees and shrubs are important flora to maintain the ecosystem of any habitat where the vegetation is rich
Number of molecular markers have been used for phylogenetic study such as random amplified polymorphic DNA (RAPD) [5], amplified fragment length polymorphism AFLP [6], restriction fragment length polymorphism (RFLP) etc
Based on available literature about the Acacia species, in the current study, we used nrDNA-ITS locus for phylogenetic study of different species of Acacia species found in the Al-Baha province
Summary
Trees and shrubs are important flora to maintain the ecosystem of any habitat where the vegetation is rich. Keywords nrDNA-ITS, Molecular Marker, Genomic DNA, PCR The number of tree species in Saudi Arabia is more than the floristic element. Acacia contains the highest number of species followed by Ficus genus. Acacia species have social and economic importance throughout the warm and tropical regions of the world.
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