Phylogenetic relationships of some economically important cereal plants based on genome characterization using molecular markers

Abstract

Genomes of 14 cereal germplasms (three barley cultivars: G124, G128, and G2000; two maize cultivars: Hybrid 10, and Hybrid 311; three rice cultivars: Sakha 101, Sakha 102, and G 182; two sorghum cultivars: white sorghum, and red sorghum; and four wheat cultivars: Sakha 95, Gemmizah 9, G 168, and Sakha 93) were characterized using two molecular markers: inter simple sequence repeat polymerase chain reaction (ISSR-PCR) and randomly amplified polymorphic DNA PCR (RAPD-PCR) to address the genetic relationships among them. Ten ISSR primers were used for fingerprinting the 14 cereal cultivars. A total of 109 ISSR markers of 400–3000 bp were scored as a result of ISSR-PCR markers. In addition, 130 RAPD markers were scored as a result of fingerprinting the same cultivars using 15 RAPD primers. ISSR-PCR and RAPD-PCR data were analyzed separately and together using the numerical taxonomy system of multivariate statistical (NTSYS-pc) program to address the genetic relationships among the 14 cereal cultivars under st...