Phylogenetic relationships of native Turkish cattle breeds using microsatellite markers

Yusuf Özşensoy,Müge Doğan,Ercan Kurar,Vahdettin Altunok,Ayşe Işik,Mehmet Ni̇zamlioğlu,Zafer Bulut

doi:10.3906/vet-1805-10

Abstract

A total of 20 microsatellite DNA markers were used for genetic characterization and determination of phylogenetic relationships of native cattle breeds of Turkey, including the Anatolian Grey (AG), Anatolian Black (AB), South Anatolian Red (SAR), East Anatolian Red (EAR), Southern Anatolian Yellow (SAY), and Zavot (ZAV). DNA samples were isolated from 271 blood samples using an organic method. Amplified polymerase chain reaction products were separated by capillary electrophoresis and genotypes were determined for 20 microsatellites. A total of 269 different alleles were determined. The lowest (7.80) and highest (10.80) mean allele numbers were observed for the ZAV and SAY populations, respectively. TGLA122 was the most polymorphic locus; however, only 7 different alleles were observed for INRA005. A total of 40 different private alleles were determined. The general FIS values were between 0.034 and 0.123. Due to the close location to the domestication center, higher genetic diversities were observed. The observed genetic diversities and the results of the phylogenetic analyses were in agreement with evolutionary history and the geographical origins of Turkish native cattle breeds.

Highlights

In order to determine the origin of breed domestications, genetic characterization and archaeological studies have been carried out
Previous archeological and molecular findings indicate that most animal breeds originated and spread from these regions to the rest of the world, especially from Anatolia to Europe [2,3,4,5,6]
The main routes of cattle coming into Europe were determined to stem from the eastern region [7]

Summary

Introduction

In order to determine the origin of breed domestications, genetic characterization and archaeological studies have been carried out. Cattle, sheep, goats, pigs, and buffalo were first domesticated in two different regions, including Southwest Asia and East Asia [1]. The oldest of these centers contains the eastern and southeastern Anatolian regions. Previous archeological and molecular findings indicate that most animal breeds originated and spread from these regions to the rest of the world, especially from Anatolia to Europe [2,3,4,5,6]. The main routes of cattle coming into Europe were determined to stem from the eastern region [7]

Objectives

Methods

Results

Conclusion