Abstract
Abstract The present study is a brief description of the morphology and molecular phylogeny of Malayanodiscoides bihamuli Lim and Furtado, 1986 infecting gill filaments of fish Notopterus chitala (Hamilton). The phylogenetic study was made of M. bihamuli and closely related taxa using DNA sequence data obtained from 28S rDNA region. Morphology as well as molecular phylogeny strongly supports the establishment of genus Malayanodiscoides and also demonstrates its close relationship with other genera of class Monogenea. In addition, the 28S secondary structure model was predicted and found to serve as a useful tool for reconstruction of optimal alignment and can be used as an additional source of data incorporating structural parameters of molecules for the study of phylogeny. Application of the secondary 28S structure data allows a more resolved and realistic picture of relationships. Results also demonstrate the phylogenetic utility of the 28S sequence secondary structure data for inferences at higher taxonomic levels.
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