Phylogenetic analysis of see-transmitted isolate of Zucchini yellow mosaic virus

O Tymchyshyn,I Budzanivska,T Shevchenko Shevchenko,I Kosenko,O Shevchenko

doi:10.17721/1728_2748.2017.74.46-50

Abstract

Zucchini yellow mosaic virus (ZYMV) remains one of the most widespread and destructive viruses affecting plants from Cucurbitaceae family in Ukraine as well as in other countries. ZYMV during the early stages of plant development can cause significant losses in yield. In current project the possibility of seed transmission of Ukrainian ZYMV isolates was tested on Cucurbita pepo plants in insect-free greenhouse. The rate was assessed by ELISA and RT-PCR. Only one isolate ZYMV-14P showed seedborne transmission with transmission rate 2,6%. This is the first detected seed-transmitted isolate in Ukraine. Phylogenetic analysis defined ZYMV-14P isolate as member of group A. This isolate was clustered with other known Ukrainian isolates and isolates from Hungary, Czech Republic, Austria and France within subgroup AI.

Highlights

Zucchini yellow mosaic virus (ZYMV) is a single-stranded positive-sense RNA virus of the family Potyviridae that can result in yellowing, severe leaf and fruit deformation, especially on plants from Cucurbitaceae family, that can reduce yields up to 94% [3]
ZYMV strains belong to three diverse phylogenetic groups (A, B and C), which have different biological and molecular properties and distribution [2]
ZYMV isolates circulating in Ukraine belong to phylogenetic group A and highly related to strains and isolates from Hungary, Czech Republic, Austria and France from subgroup AI [9]

Summary

Introduction

Zucchini yellow mosaic virus (ZYMV) is a single-stranded positive-sense RNA virus of the family Potyviridae that can result in yellowing, severe leaf and fruit deformation, especially on plants from Cucurbitaceae family, that can reduce yields up to 94% [3]. Since its discovery this virus has spread rapidly and achieved the global distribution within two decades of its discovery [4]. C. pepo seeds and measured seed transmission of ZYMV isolates by visual observation, ELISA and RT-PCR.

Results and discussion

Zucchini Cucurbita pepo

Conclusions