Abstract
Phylogenetic relationships of five taxa of Clivia, one probable new species plus four recognized species, and three outgroup species were studied using sequences of the nuclear ribosomal 5S non-transcribed spacer and the internal transcribed spacer (ITS) of 45S rDNA. Analysis of the data sets separately generated some well-supported groupings and congruent phylogenies. Clivia miniata and C. gardenii are closely related. ‘Robust Gardenii’, the putative new species, is a sister clade of this group. Clivia nobilis is distantly related to these three taxa and C. caulescens occupies an intermediate position between the two groups. Chromosome locations and distribution patterns of the 5S nuclear ribosomal gene in the species of Clivia were investigated using fluorescencein situ hybridization (FISH). In all species, only one pair of 5S rDNA signals was observed. These were located on the short arm of chromosome 8, at the position of the interstitial C-bands. The phylogenies obtained from the DNA sequences together with the chromosome data accumulated here and previously published information on the location of the 45S rDNA sites have been used to postulate evolutionary trends in Clivia chromosomes.
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