Phylogenetic Affinities of Indian Apple Snails: An Insight into the Tibetan Tectonic Terranes

Abstract

Gastropods belong to a hyper-diverse clade of molluscs and encompass limpets, slugs and snails. Interestingly, gastropods have effectively colonized land, freshwaters and marine habitats. However, the relationships among and within its constituent taxa remained in flux for decades due to delimitation in their morphological and anatomical features and hence molecular approach has taken a lead. An understanding into the history of Tibetan tectonic terranes is attempted in this article to derive through the phylogeny of Pila. In our study, we have re-evaluated the relationships within Ampullariidae by collecting the species of Pila globosa and Pila virens (India) and analyzed them comparing with 18S rDNA sequences from public domain. Obtained sequences are evaluated through Maximum Likelihood method in MEGA v 5 which clearly established a single cluster for the three individual taxons namely P. globosa with high bootstrap support. Pila virens showed a paraphyletic cluster with Pila ovata. A low bootstrap value of a few species of Pila possibly inferred rapid speciation among them. The novel observation in the present study is that P. virens forming a secondary cluster with the species from Vietnam namely Pila polita, Pila conica and Pila ampullacea has authenticated that the south India and Vietnam are in the same latitude namely 9–20°N, supporting the paleomagnetic data which unravelled that India collided with Asia around 57 mya in the Coenozoic era, whereas ampullariids must have been in co-existence since the Cambrian Period indicating their long evolutionary affinity and zoo-geographical relationship.