Phyllanthuse emblica polyphenols: optimization of high-voltage pulsed electric field assisted extraction, an antioxidant and anti-inflammatory effects in vitro

Abstract

BackgroundThe polyphenols extraction of Phyllanthus emblica is primarily carried out using organic solvents, and assisted by physical fields such as ultrasound and microwave for extraction. High voltage pulsed electric field technology (PEF) is a non-thermal processing technology that has high efficiency and minimal damage to thermosensitive substances. PEF has been applied to plant extraction in many studies, however, the extraction of polyphenols from Phyllanthus emblica using the PEF has still not been reported; ObjectiveThis study explores the optimal extraction process of polyphenols from Phyllanthus emblica using the PEF, and investigates its relaxation and anti-wrinkle based on anti-oxidation and anti-inflammatory experiment, in order to develop a Phyllanthus emblica extract with substantial efficacy; Materials and MethodsThe method of Phyllanthus emblica extract using PEF is established, and compared with a traditional extraction method. The experimental conditions, such as electric field intensity (0.5~6.0kV/cm), pulse times (20~120), extraction time (0~60min) and material concentration (0.5%~3%), are investigated and optimized using orthogonal experiments; Resultsthe polyphenols in the Phyllanthus emblica extract were highest at the electric field intensity of 5kV / cm, 120 pulses, extraction time of 30minutes, and 2% material concentration. The PEFcontained more polyphenols than the conventional water extraction and ultrasound-assisted extraction. The Phyllanthus emblica extract had substantial antioxidant and anti-inflammatory effects, with a good clearance effect on DPPH ( IC50 of 1.82%) and ABTS ( IC50 of 1.80%) radicals. At the Phyllanthus emblica extract concentration of 1.25%, inflammatory factors (TNF-α) were reduced by 47.08%; and ConclusionThe PEF is a leading-edge and promising method for preparing Phyllanthus emblica extracts.