Abstract

The photothermal (PT) effect is applied for imaging and quantitative studies of samples with low optical contrast or low absorbance. The dual pulsed laser setup combined with phase-contrast illumination of a sample by probe laser provides visualisation of temperature field or absorbing structure of a sample. The spatial resolution is about 0.5 /spl mu/m. The sensitivity of the PT technique provides imaging of live (non-stained) white blood cells (WBCs). An automated system and software was developed for optical and PT image acquisition, processing and analysis at the rate 1 cell per second. The system was tested during normal/abnormal blood cells (red blood cells, WBCs) studies. >

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