Photosynthetic performance along a light gradient as related to leaf characteristics of a naturally occurring Cypripedium flavum

Abstract

Photosynthesis, leaf structure, nitrogen content and nitrogen allocation in photosynthetic functions of Cypripedium flavum were studied in a naturally varying light regime. Light-saturated leaf net photosynthetic rate (A max) was strongly correlated with leaf dry mass per area (LMA), mesophyll conductance (g (m)) and area-based leaf nitrogen content (N area), with all variables increasing with increasing irradiance. Such coordinate variation of all these parameters illustrates the plastic response of leaf structure to high light (HL). Leaf N area was greater under HL than in low light (LL). The fractions of leaf nitrogen partitioning in carboxylation (P (R)) and bioenergetics (P (B)) were positively related to LMA. In contrast, P (R) and P (B) decreased with increasing mass-based leaf nitrogen content (N mass). However, no correlation was found between leaf nitrogen investment in light harvesting (P (L)) and either LMA or N mass. Like maximum rate of carboxylation (V cmax) and electron transport (J max), the J max/V cmax ratio, which was strongly correlated to LMA, also increased significantly with irradiance. Under HL, leaf maximum photosynthetic nitrogen efficiency (ANUE) and intrinsic water use efficiency (WUE) were greater than in LL conditions, despite a small difference in WUE. This suggests that a functional balance in the photosynthetic machinery favors leaf photosynthetic plasticity of C. flavum in response to different light conditions. Given an ample soil nitrogen supply, C. flavum may offset its susceptibility to HL by efficient nitrogen use and higher stomatal and mesophyll conductance against photoinhibition so as to keep leaf photosynthesis positive.