Abstract
We report a photoresist-based microfluidic cell sorting system for genitourinary cancer based on photodynamic analysis of urine and its potential for a clinical application. In the system, genitourinary cancer cells in urine are detected using 5-aminolevulinic acid (ALA)-induced fluorescence, and then sorted and concentrated for the gene alteration analysis. In order to achieve sensitive fluorescence detection, autofluorescence in detection area was minimized by thinning photoresist membrane of the microchannel and inclining the optical angle of detection system. Our experimental results demonstrate that the ALA-based fluorescence detection assay using the proposed device is promising tool for early diagnosis of genitourinary cancers.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
