Abstract
Abstract— The photoreaction of the acidified form of bacteriorhodopsin and its 9‐cis derivative was studied by low temperature spectroscopy.A short exposure of the acidified form of bacteriorhodopsin, which was prepared by adding 2 mM HC1 to purple membrane suspension in 67% glycerol at 0°C, to red light at – 72°C resulted in the blue‐shift of the spectrum. The feature of the shift was very similar to that accompanied by the formation of stable 9‐cis acidified form of bacteriorhodopsin at 0°C, but only 13‐cis‐ and all‐trans‐retinals were found in the extract from this product. No blue‐shifted product was found on irradiation at – 190°C.Irradiation of the 9‐cis form of acidified bacteriorhodopsin at ‐72°C with blue light caused the isomerization of its 9‐cis‐retinylidene chromophore to 13‐cis and all‐trans forms without a significant spectral change. It became greater only after the sample was warmed above – 24°C. These results indicate the presence of the light‐induced product which has trans configuration on the 9‐10 double bond and exhibits the 9‐cis type spectrum.
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