PHOTOPHYSICAL PROPERTIES OF INDOTRICARBOCYANINE DYES DURING COMPLEXATION WITH SERUM ALBUMIN

Abstract

The photophysical properties of indotricarbocyanine dyes upon complexation with serum albumin have been studied, and the technique for controlling their formation using electrophoresis has been optimized. In connection with the degradation of dye molecules under the action of acids, the search for the area of localization of the dye under study on the surface of the gel plate was carried out by recording the fluorescence spectra of the dye before protein fixation and visualization followed by the completion of the protocol for obtaining electrophoregrams. To minimize the possible influence of the luminescence of the gel components, the excitation was carried out by the radiation of a semiconductor laser with a wavelength of 684 nm, which initiates the fluorescence of the studied dyes. It was established that the position of the maxima and the half-width of the fluorescence spectra of dyes with an orthophenylene bridge in the conjugation chain in the regions of the electropherogram corresponding to the localization of albumin coincide with the characteristics of the emission of dyes in initial solutions with albumin, which makes it possible to reveal the formation of covalently bound complexes of dye molecules with albumin.