PHOTODYNAMIC THERAPY FOR LIVER CIRRHOSIS (EXPERIMENTAL STUDY)

Abstract

Aim. To study the effects of photodynamic therapy for liver cirrhosis in experiment. Material and Methods. Liver cirrhosis was induced in 44 white rats-males Wistar rats using CCl4 in the experiment. Photolon at a dose of 3.0 mg/kg was intravenously introduced (tail vein) in experimental groups of animals in 1 day after the last injection of CCl4. In 3 hours laser irradiation of liver was performed at a dose of 10 j/cm2 with a wavelength of 670 nm. Morphological examination of liver tissue, electron microscopy, biochemical analysis of blood, determination of free amino acids in blood plasma were carried out. Results. There was reduction of connective tissue in liver parenchyma after photodynamic therapy. Hepatocytes' alternative changes and their lipid infiltration were drastically reduced. Infiltration corresponded to <1.0 by Hornboll. Protein biosynthesis was activated. Ito cells was in passive state that indicated on positive effect of photodynamic therapy on the processes of collagen formation. Use of photodynamic therapy decresed total bilirubin in 2.16 times, alanine aminotransferase – 2.96 times, De Ritis coefficient – 2 times, alkaline phosphatase – 4.4 times, lactate dehy-drogenase – 3.1 times, gamma glutamyltranspeptidase – 2.86 times. Total level of amino acids decreased in 1.43 times, proteinogenic amino acids – in 1.43 times, non-essential – in 1.53 times, aromatic amino acids – in 1.72 times in experimental animals than in those with liver cirrhosis at the same time without treatment. Level of proline and hydroxyproline was reduced by 55% and 52% and it was significantly lower than in rats without treatment in 1.77 and 1.63 times, respectively. Conclusion. The experimental study indicates on advisability to study this technique in clinical conditions in liver cirrhosis patients.