Abstract

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Highlights

  • The aim of this experiment was to study the changes in free amino acid (AA) concentration in blood plasma of dairy cows developing hepatic steatosis after parturition and to establish the diagnosis by means of Fischer’s index

  • Our results were comparable to the values presented by other authors: Broderick et al (1974) 1770 μmol.l-1 Rogers et al (1987) 1880 – 2000 μmol.l-1 during the observation of similar spectrum of plasma free amino acids

  • We have examinated above all Fischer’s index (BCAA/AAA), total free amino acids in blood plasma and EAA/NEAA ratio as related to hepatic steatosis

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Summary

Introduction

The aim of this experiment was to study the changes in free amino acid (AA) concentration in blood plasma of dairy cows developing hepatic steatosis after parturition and to establish the diagnosis by means of Fischer’s index. Important is the so-called Fischer’s index which represents molar ratio of BCAA/AAA (BCAA – branched chain amino acid = valine, leucine, isoleucine; AAA – aromatic amino acid = tyrosine, phenylalanine) This ratio is based on different metabolic routes of these AA groups. Fa lloon et al (1988) studied the plasma free AA concentration in relation to the metabolic disturbances in cattle These authors recommend the study of mutual concentration ratio to absolute concentration of simple free AA; Fischer’s index (BCAA/AAA) can be used as an indicator of liver impairment, independent of animal species, diet, and/or the cause of liver impairment. Cattle with calcium concentration under 1.6 mmol.l-1 had higher values of alanine, glycine, glutamine, taurine, proline, and lower values of citrulline, isoleucine and valine

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